The present investigation aimed at development and validation of an analytical method for the estimation of Gemcitabine Hydrochloride based on Ultra-violet spectrophotometry. The reported methods of analysis include the chromatographic methods such as HPLC (1) RP-HPLC (2-4), Ion pair HPLC (5), and LC-MSMS (6). Chromatographic methods are tedious, time consuming and are not suitable for routine analysis. However, Ultraviolet spectophotometric methods which are simple and fast are ideal for routine analysis. Gemcitabine Hydrochloride exhibits a sharp peak at 268 nm when scanned in the Ultraviolet region. Thus, it was selected as the analytical wave-length. The developed method was found to be simple, sensitive, accurate, precise and specific as evidenced by non-interference from the excipients present in the marketed injection. Linearity was observed in the range of 5µg/ml to 35 µg/ml. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.384 µ/ml & 1.163 µ/ml respectively.
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